Fate of primordial germ cells in the transplanted hind gut of mouse embryos.
نویسنده
چکیده
During normal development of the mouse embryo, primordial germ cells (PGCs) differentiate in the root of the allantois and in the hind region of the embryo, then pass to the hind gut and through the mesentery to reach the germinal ridges (Chiquoine, 1954; Bennett, 1956; Mintz & Russell, 1957; Mintz, 1957; Ozdzenski, 1967). The number of PGCs increases greatly during migration (Chiquoine, 1954; Mintz & Russell, 1957). After penetrating into the gonads PGCs continue to divide mitotically for a certain time, then undergo changes which differ in each sex: the meiotic prophase begins in the ovaries (Brambell, 1927; Borum, 1961), gonial divisions are arrested in the testes and the chromatin in the nuclei of the germ cells undergoes characteristic dispersion. The behaviour of germ cells in the male embryos of the mouse is similar to that described by Clermont & Perey (1957) and Beaumont & Mandl (1963) in the rat. The present study has been undertaken in order to investigate the fate of PGCs which were prevented from penetrating into the germinal ridges. In particular we were interested in the question as to whether PGCs remaining outside the gonad are able to initiate differentiation into definitive germ cells, i.e. to enter into meiotic prophase. As an object of study the hind gut of 9-day-old embryos (= 10th day of pregnancy) was chosen on account of the considerable number of PGCs present in it at this time, and the ease with which it can be dissected out without risk of the rudiments of germinal ridges being included in the transplant.
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Behaviour of mouse primordial germ cells in the chick embryo.
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عنوان ژورنال:
- Journal of embryology and experimental morphology
دوره 22 3 شماره
صفحات -
تاریخ انتشار 1969